ADAM10 Antibody (CL13814)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24579
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL13814
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was designed to target the recombinant protein ADAM10, with the precise immunogen sequence being withheld for proprietary reasons.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for ADAM10 Antibody (CL13814)
Immunohistochemistry-Paraffin: ADAM10 Antibody (CL13814) [NBP3-24579]
Staining of human prostate shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: ADAM10 Antibody (CL13814) [NBP3-24579]
Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: ADAM10 Antibody (CL13814) [NBP3-24579]
Staining of human fallopian tube shows strong cytoplasmic and membranous positivity in glandular cells.
Applications for ADAM10 Antibody (CL13814)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ADAM10
Long Name
A Disintegrin and Metalloprotease-like Domain 10
Alternate Names
CD156c, Kuzbanian, MADM
Gene Symbol
ADAM10
Additional ADAM10 Products
Product Documents for ADAM10 Antibody (CL13814)
Product Specific Notices for ADAM10 Antibody (CL13814)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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