ADAM12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-889

Novus Biologicals, part of Bio-Techne
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NB300-889
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ADAM12 Antibodies »

Product Specifications

Immunogen

Peptide with sequence AARPLPVSPARALC corresponding to N-Terminus according to NP_003465.3, NP_067673.2.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23124962).

Specificity

This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ADAM12 Antibody

Immunocytochemistry/ Immunofluorescence: ADAM12 Antibody [NB300-889]

Immunocytochemistry/ Immunofluorescence: ADAM12 Antibody [NB300-889]

Immunocytochemistry/Immunofluorescence: ADAM12 Antibody [NB300-889] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
Immunohistochemistry-Paraffin: ADAM12 Antibody [NB300-889]

Immunohistochemistry-Paraffin: ADAM12 Antibody [NB300-889]

Immunohistochemistry-Paraffin: ADAM12 Antibody [NB300-889] - Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: ADAM12 Antibody [NB300-889]

Flow Cytometry: ADAM12 Antibody [NB300-889]

Flow Cytometry: ADAM12 Antibody [NB300-889] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for ADAM12 Antibody

Application
Recommended Usage

Flow Cytometry

10ug/ml

Immunocytochemistry/ Immunofluorescence

10ug/ml

Immunohistochemistry

3.7 ug/ml

Immunohistochemistry-Paraffin

3.7 ug/ml

Peptide ELISA

Detection limit 1:64000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ADAM12

This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis.

Long Name

A Disintegrin and Metalloprotease-like Domain 12

Alternate Names

Meltrin alpha

Entrez Gene IDs

8038 (Human)

Gene Symbol

ADAM12

UniProt

O43184

Additional ADAM12 Products

Product Documents for ADAM12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ADAM12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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