ADAM8 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1017
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-QRKQGAGAPTAP corresponding to C-Terminus according to NP_001100.3, NP_001157962.1.
Localization
Cell Membrane
Specificity
This antibody is expected to recognise isoform 1 and 3 (NP_001100.3; NP_001157962.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ADAM8 Antibody
Western Blot: Goat Polyclonal ADAM8 Antibody [NB100-1017] -
Western Blot: Goat Polyclonal ADAM8 Antibody [NB100-1017] - (1µg/ml) staining of Caco-2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western Blot: Goat Polyclonal ADAM8 Antibody [NB100-1017] -
Western Blot: Goat Polyclonal ADAM8 Antibody [NB100-1017] - (1µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Flow Cytometry: Goat Polyclonal ADAM8 Antibody [NB100-1017] -
Flow Cytometry: Goat Polyclonal ADAM8 Antibody [NB100-1017] - Flow cytometric analysis of paraformaldehyde fixed Caco-2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for ADAM8 Antibody
Application
Recommended Usage
ELISA
Detection limit 1:16000
Flow Cytometry
10 ug/ml
Immunohistochemistry
6-8 ug/ml
Immunohistochemistry-Paraffin
6-8 ug/ml
Western Blot
1 - 3 ug/ml
Application Notes
Approx. 90kDa band observed in lysates of cell line Caco-2, approx. 80kDa in Human Duodenum lysates and approx. 85kDa in preliminary testing of MCF7 cell lysate (calculated MW of 88.8kDa according to NP_001100.3 and 78.8kDa according to NP_001157962.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ADAM8
Long Name
A Disintegrin and Metalloprotease-like Domain 8
Alternate Names
CD156a, MS2
Gene Symbol
ADAM8
UniProt
Additional ADAM8 Products
Product Documents for ADAM8 Antibody
Product Specific Notices for ADAM8 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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