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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Summary for ADAM9 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 400 to 450 of human ADAM Metallopeptidase Domain 9 using the numbering given in entry NP_003807.1 (GeneID 8754).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ADAM9 Antibody

Western Blot: ADAM9 Antibody [NBP2-32260]

Western Blot: ADAM9 Antibody [NBP2-32260]

Western Blot: ADAM9 Antibody [NBP2-32260] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-ADAM9 antibody NBP2-32260 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: ADAM9 Antibody [NBP2-32260]

Immunoprecipitation: ADAM9 Antibody [NBP2-32260]

Immunoprecipitation: ADAM9 Antibody [NBP2-32260] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-ADAM9 antibody NBP2-32260 used for IP at 6 ug per reaction. ADAM9 was also immunoprecipitated by rabbit anti-ADAM9 antibody BL16249. For blotting immunoprecipitated ADAM9, NBP2-32260 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for ADAM9 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:10000 - 1:25000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ADAM9

ADAM9 encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Two alternative splice variants have been identified, encoding distinct isoforms.

Long Name

A Disintegrin and Metalloprotease-like Domain 9

Alternate Names

MCMP, MDC9, Meltrin gamma

Gene Symbol

ADAM9

Additional ADAM9 Products

Product Documents for ADAM9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ADAM9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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