Adenosylhomocysteinase/AHCY Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48817

Novus Biologicals, part of Bio-Techne
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NBP2-48817
NBP2-48817-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Adenosylhomocysteinase/AHCY Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: HPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Adenosylhomocysteinase/AHCY Antibody

Western Blot: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817]

Western Blot: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817]

Western Blot: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817] - Analysis in human cell line CACO-2 and human cell line HeLa.
Immunocytochemistry/ Immunofluorescence: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817]

Immunocytochemistry/ Immunofluorescence: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817]

Immunocytochemistry/Immunofluorescence: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817] - Staining of human cell line CACO-2 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817]

Immunohistochemistry-Paraffin: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817]

Immunohistochemistry-Paraffin: Adenosylhomocysteinase/AHCY Antibody [NBP2-48817] - Staining of human Small intestine shows moderate cytoplasmic positivity in glandular cells.
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Applications for Adenosylhomocysteinase/AHCY Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Adenosylhomocysteinase/AHCY

S-adenosylhomocysteine hydrolase catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family.

Alternate Names

AdoHcyase, AHCY, SAHH

Gene Symbol

AHCY

Additional Adenosylhomocysteinase/AHCY Products

Product Documents for Adenosylhomocysteinase/AHCY Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Adenosylhomocysteinase/AHCY Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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