Adenylate Kinase 1 Antibody (3A6-1F5)

Novus Biologicals, part of Bio-Techne | Catalog # H00000203-M06

Novus Biologicals, part of Bio-Techne
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H00000203-M06
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3A6-1F5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Adenylate Kinase 1 Antibodies »

Product Specifications

Immunogen

AK1 (AAH01116, 1 a.a. ~ 194 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEEKLKKTNIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRSEVSSGSARGKKLSEIMEKGQLVPLETVLDMLRDAMVAKVNTSKGFLIDGYPREVQQGEEFERRIGQPTLLLYVDAGPETMTQRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDSVFSQVCTHLDALK

Specificity

AK1 - adenylate kinase 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Adenylate Kinase 1 Antibody (3A6-1F5)

Western Blot: Adenylate Kinase 1 Antibody (3A6-1F5) [H00000203-M06]

Western Blot: Adenylate Kinase 1 Antibody (3A6-1F5) [H00000203-M06]

Western Blot: Adenylate Kinase 1 Antibody (3A6-1F5) [H00000203-M06] - Analysis of AK1 expression in transfected 293T cell line by AK1 monoclonal antibody (M06), clone 3A6-1F5. Lane 1: AK1 transfected lysatE (21.6 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody (3A6-1F5) [H00000203-M06]

Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody (3A6-1F5) [H00000203-M06]

Immunocytochemistry/Immunofluorescence: Adenylate Kinase 1 Antibody (3A6-1F5) [H00000203-M06] - Analysis of monoclonal antibody to AK1 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: Adenylate Kinase 1 Antibody (3A6-1F5) [H00000203-M06]

Western Blot: Adenylate Kinase 1 Antibody (3A6-1F5) [H00000203-M06]

Western Blot: Adenylate Kinase 1 Antibody (3A6-1F5) [H00000203-M06] - Western blot analysis of AK1 over-expressed 293 cell line. Lane 2 cotransfected with AK1 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000203-M06. GAPDH ( 36.1 kDa ) used as loading control.
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Applications for Adenylate Kinase 1 Antibody (3A6-1F5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein and cell lysate for WB. It has also been used for IF, RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Adenylate Kinase 1

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Long Name

Adenylate kinase isoenzyme 1

Alternate Names

AK 1, AK1, EC 2.7.4.3, EC 2.7.4.6, Myokinase

Entrez Gene IDs

203 (Human)

Gene Symbol

AK1

OMIM

103000 (Human)

UniProt

AAH01116 (Human)

Additional Adenylate Kinase 1 Products

Product Documents for Adenylate Kinase 1 Antibody (3A6-1F5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Adenylate Kinase 1 Antibody (3A6-1F5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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