Adenylate Kinase 1 Antibody (OTI4A1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47655

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4A1

Concentration

0.89 mg/ml

View all Adenylate Kinase 1 Antibodies »

Product Summary for Adenylate Kinase 1 Antibody (OTI4A1)

Immunogen

Full length human recombinant protein of human AK1 (NP_000467) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens adenylate kinase 1 (AK1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adenylate Kinase 1 Antibody (OTI4A1)

Western Blot: Adenylate Kinase 1 Antibody (4A1) [NBP1-47655] Analysis of extracts (35ug) from 9 different cell lines by using anti-Adenylate Kinase 1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody (OTI4A1) [NBP1-47655]

Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody (OTI4A1) [NBP1-47655]

Immunocytochemistry/Immunofluorescence: Adenylate Kinase 1 Antibody (4A1) [NBP1-47655] - Immunofluorescent staining of HT29 cells using anti-Adenylate Kinase 1 mouse monoclonal antibody.
Flow Cytometry: Adenylate Kinase 1 Antibody (OTI4A1) [NBP1-47655]

Flow Cytometry: Adenylate Kinase 1 Antibody (OTI4A1) [NBP1-47655]

Flow Cytometry: Adenylate Kinase 1 Antibody (4A1) [NBP1-47655] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Adenylate Kinase 1 antibody, and then analyzed by flow cytometry.
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Applications for Adenylate Kinase 1 Antibody (OTI4A1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.89 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Adenylate Kinase 1

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Long Name

Adenylate kinase isoenzyme 1

Alternate Names

AK 1, AK1, EC 2.7.4.3, EC 2.7.4.6, Myokinase

Entrez Gene IDs

203 (Human)

Gene Symbol

AK1

Additional Adenylate Kinase 1 Products

Product Documents for Adenylate Kinase 1 Antibody (OTI4A1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Adenylate Kinase 1 Antibody (OTI4A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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