Adenylate Kinase 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87401

Novus Biologicals, part of Bio-Techne
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NBP1-87401
NBP1-87401-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat, Rhesus Macaque

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Adenylate Kinase 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EKGQLVPLETVLDMLRDAMVAKVNTSKGFLIDGYPREVQQGEEFERRIGQPTLLLYVDAGPETMTQRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGS

Reactivity Notes

Rhesus macaque reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adenylate Kinase 1 Antibody

Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401]

Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401]

Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining in human fallopian tube and small intestine tissues using anti-AK1 antibody. Corresponding AK1 RNA-seq data are presented for the same tissues.
Western Blot: Adenylate Kinase 1 Antibody [NBP1-87401]

Western Blot: Adenylate Kinase 1 Antibody [NBP1-87401]

Western Blot: Adenylate Kinase 1 Antibody [NBP1-87401] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody [NBP1-87401]

Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody [NBP1-87401]

Immunocytochemistry/Immunofluorescence: Adenylate Kinase 1 Antibody [NBP1-87401] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
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Applications for Adenylate Kinase 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25 - 2 ug/mL

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/mL
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-87401 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2), 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Adenylate Kinase 1

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Long Name

Adenylate kinase isoenzyme 1

Alternate Names

AK 1, AK1, EC 2.7.4.3, EC 2.7.4.6, Myokinase

Entrez Gene IDs

203 (Human)

Gene Symbol

AK1

UniProt

P00568 (Human)

Additional Adenylate Kinase 1 Products

Product Documents for Adenylate Kinase 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Adenylate Kinase 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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