Adenylate Kinase 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15295

Novus Biologicals, part of Bio-Techne
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NBP2-15295
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (96%), Canine (97%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Adenylate Kinase 1 Antibodies »

Product Summary for Adenylate Kinase 1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Adenylate kinase 1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (86%), Xenopus tropicalis (80%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adenylate Kinase 1 Antibody

Western Blot: Adenylate Kinase 1 Antibody [NBP2-15295]

Western Blot: Adenylate Kinase 1 Antibody [NBP2-15295]

Western Blot: Adenylate Kinase 1 Antibody [NBP2-15295] - Wild-type (WT) and Adenylate kinase 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Adenylate Kinase 1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP2-15295]

Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP2-15295]

Immunohistochemistry-Paraffin: Adenylate Kinase 1 Antibody [NBP2-15295] - Ca922 xenograft.
Western Blot: Adenylate Kinase 1 Antibody [NBP2-15295]

Western Blot: Adenylate Kinase 1 Antibody [NBP2-15295]

Western Blot: Adenylate Kinase 1 Antibody [NBP2-15295] - Sample (50 ug of whole cell lysate) A: Mouse brown adipose tissue 12% SDS PAGE; antibody diluted at 1:1000.
View 1 more images

Applications for Adenylate Kinase 1 Antibody

Application
Recommended Usage

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Adenylate Kinase 1

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme. [provided by RefSeq]

Long Name

Adenylate kinase isoenzyme 1

Alternate Names

AK 1, AK1, EC 2.7.4.3, EC 2.7.4.6, Myokinase

Gene Symbol

AK1

UniProt

P00568 (Human)

Additional Adenylate Kinase 1 Products

Product Documents for Adenylate Kinase 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Adenylate Kinase 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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