Adenylosuccinate Synthase Antibody (JE64-42)

Immunohistochemistry: Adenylosuccinate Synthase Antibody (JE64-42) [NBP3-32005]

Immunohistochemistry: Adenylosuccinate Synthase Antibody (JE64-42) [NBP3-32005] - Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Adenylosuccinate Synthase antibody (NBP3-32005) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32005) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Adenylosuccinate Synthase Antibody (JE64-42) [NBP3-32005]

Western Blot: Adenylosuccinate Synthase Antibody (JE64-42) [NBP3-32005] - Western blot analysis of Adenylosuccinate Synthase on different lysates with Rabbit anti-Adenylosuccinate Synthase antibody (NBP3-32005) at 1/500 dilution.

Lane 1: 293T cell lysate
Lane 2: U87MG cell lysate
Lane 3: HL-60 cell lysate
Lane 4: Mouse kidney tissue lysate (20 ug/Lane)

Lysates/proteins at 10 ug/Lane.

Predicted band size: 50 kDa
Observed band size: 50 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32005) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.