AF10 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25268
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for AF10 Antibody
Immunogen
This antibody has been engineered to specifically recognize the recombinant protein AF10 using the following amino acid sequence: IVGALNGVMQTPVTMSQNPTPLTHTTVPPNATHPMPATLTNSASGLGLLSDQQRQILIHQQQFQQLLNSQQLTPVHRHPHFTQLPPTHFSPSM
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AF10 Antibody
![AF10 Antibody Immunohistochemistry-Paraffin: AF10 Antibody [NBP3-25268]](https://resources.bio-techne.com/images/products/nbp3-25268_rabbit-af10-pab-2012202314563421.jpg "Immunohistochemistry-Paraffin: AF10 Antibody [NBP3-25268]")
Immunohistochemistry-Paraffin: AF10 Antibody [NBP3-25268]
Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.![AF10 Antibody Western Blot: AF10 Antibody [NBP3-25268]](https://resources.bio-techne.com/images/products/nbp3-25268_rabbit-af10-pab-1912202316102317.jpg "Western Blot: AF10 Antibody [NBP3-25268]")
Western Blot: AF10 Antibody [NBP3-25268]
Lane 1: Marker [kDa] 250,130,95,72,55,36,28,17,10Lane 2: RT-4
Lane 3: U-251MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
Applications for AF10 Antibody
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 µg/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AF10
Alternate Names
AF10MGC75086, ALL1-fused gene from chromosome 10 protein, DKFZp686E10210, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 10, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 10, protein AF-10, type I AF10 protein
Gene Symbol
MLLT10
Additional AF10 Products
Product Documents for AF10 Antibody
Product Specific Notices for AF10 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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