AFAP Antibody (OTI2G9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46498
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI2G9
Concentration
1 mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 100-386 of human AFAP1 (NP_940997) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
80.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for AFAP Antibody (OTI2G9)
Western Blot: AFAP Antibody (OTI2G9) [NBP2-46498]
Western Blot: AFAP Antibody (2G9) [NBP2-46498] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP.Immunohistochemistry: AFAP Antibody (OTI2G9) [NBP2-46498]
Immunohistochemistry: AFAP Antibody (2G9) [NBP2-46498] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)Immunohistochemistry: AFAP Antibody (OTI2G9) [NBP2-46498]
Immunohistochemistry: AFAP Antibody (2G9) [NBP2-46498] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)Applications for AFAP Antibody (OTI2G9)
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AFAP
Long Name
Actin Filament-associated Protein, 110 kDa
Alternate Names
AFAP-110, AFAP1
Gene Symbol
AFAP1
Additional AFAP Products
Product Documents for AFAP Antibody (OTI2G9)
Product Specific Notices for AFAP Antibody (OTI2G9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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