AFAP: Proteins and Enzymes

AFAP (actin filament associated protein; also known as AFAP-110) is an adaptor protein that regulates actin filament integrity. It is both multimodular and multimeric, and is known to simultaneously bind actin and cytosolic kinases. As a homomultimer, AFAP binds and separates actin filaments, preventing actin cross-linking. Upon binding to kinases, AFAP is destabilized, promoting actin polymerization. Human AFAP is 730 amino acids in length, and contains SH3 and SH2 binding motifs, two pleckstrin homology domains, and a Leu-zipper/actin binding region.