Aflatoxin B1 Antibody (6A10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-443

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, All Species

Cited:

Human, Mouse, Rat, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Dot Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

CoIP, Cytometric Bead Assay Standard, ELISA, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6A10

Format

BSA Free

Concentration

1.0 mg/ml

View all Aflatoxin B1 Antibodies »

Product Specifications

Immunogen

Aflatoxin B1 DNA (against the midazole ring-opened persistent form of the major N-7 guanine adduct of AFB1).

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Aflatoxin B1 Antibody (6A10) - BSA Free

Immunohistochemistry: Aflatoxin B1 Antibody (6A10) - BSA Free [NB600-443]

Immunohistochemistry: Aflatoxin B1 Antibody (6A10) - BSA Free [NB600-443]

Immunohistochemistry: Aflatoxin B1 Antibody (6A10) [NB600-443] - Staining of positive (A) and negative (B) tumor tissues for AFB, -DNA adducts. Photos courtesy of Dr. Regina Santella.
Dot Blot: Aflatoxin B1 Antibody (6A10) [NB600-443] - HCT-8 cells stably-transfected with empty vector or shCYP3A5 were treated with DMSO or 10 mM AFB1 for 72 h. AFB1 DNA adduct (6A10) were detected using immunodot-blot assay. DNA adduct measured by multi gauge software (bottom panel), respectively. Image collected and cropped by CiteAb from the following publication (oncotarget.com/article/8914/text/), licensed under a CC-BY license.
Aflatoxin B1 Antibody (6A10) - BSA Free

ELISA: Aflatoxin B1 Antibody (6A10) - BSA Free [NB600-443] -

AFB1-DNA adducts formation in AFB1-treated SMMC-7721 cells with overexpression of miR-429 (see Section 2). Levels of AFB1-DNA adducts were tested using comparative ELISA. Data were analyzed from three independent tests using one-way ANOVA with Bonferroni corrections. *P < 0.05.
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Applications for Aflatoxin B1 Antibody (6A10) - BSA Free

Application
Recommended Usage

Dot Blot

reported in scientific literature (PMID 19524575)

ELISA

5 ug/ml

Immunocytochemistry/ Immunofluorescence

1:100. Use reported in scientific literature (PMID 24114584)

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 24391771)

Western Blot

1:500-1:2000

Reviewed Applications

Read 2 reviews rated 4.5 using NB600-443 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aflatoxin B1

Aflatoxins (AF) are a group of harmful fungal toxins commonly found as grains/groundnuts contaminants and the most abundant congeners of AF, aflatoxin B1 (AFB1) is the most toxic among AFs which is produced by Aspergillus flavus, A. parasiticus etc. Humans and domestic animals can be exposed to aflatoxins by foods and approximately 25% of the world's food supply is annually contaminated with mycotoxins indicating the widespread prevalence of this public health problem. AFB1, a potent hepatotoxin/hepatocarcinogen, undergoes metabolism catalyzed by mixed function oxygenases of hepatic cytochrome P-450 enzyme system to produce a highly reactive intermediate, aflatoxin B1-8,9 epoxide that binds to necleophilic sites in hepatocyte DNA to form a number of adducts. The reactive aflatoxin B1-8,9 epoxide attracts guanine residues in double stranded DNA and the adducts formation leads to initiation of hepatocarcinogenesis. ROS generated during aflatoxins metablosm are considered to be main culprit for hepatotoxicity. The AFB1-8,9-epoxide can further be converted to AFB1-8,9-diol that specifically binds to lysine in albumin and forms AFB1-lysine adducts (AFB-Lys), which has been validated as a biomarker of human aflatoxins exposure.

Alternate Names

AFB1, AFB1-AR1, Aldoketoreductase 7

Additional Aflatoxin B1 Products

Product Documents for Aflatoxin B1 Antibody (6A10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aflatoxin B1 Antibody (6A10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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