Aflatoxin B1 Antibody (6A10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80563

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, All Species

Applications

Dot Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6A10

Format

Azide and BSA Free

Concentration

1.5 mg/ml

View all Aflatoxin B1 Antibodies »

Product Specifications

Immunogen

Aflatoxin B1 DNA (against the midazole ring-opened persistent form of the major N-7 guanine adduct of AFB1).

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Aflatoxin B1 Antibody (6A10) - Azide and BSA Free

Immunohistochemistry: Aflatoxin B1 Antibody (6A10) - Azide and BSA Free [NBP2-80563]

Immunohistochemistry: Aflatoxin B1 Antibody (6A10) - Azide and BSA Free [NBP2-80563]

Immunohistochemistry: Aflatoxin B1 Antibody (6A10) - Azide and BSA Free [NBP2-80563] - Staining of positive (A) and negative (B) tumor tissues for AFB, -DNA adducts. Photos courtesy of Dr. Regina Santella. Image from the standard format of this antibody.

Applications for Aflatoxin B1 Antibody (6A10) - Azide and BSA Free

Application
Recommended Usage

ELISA

5 ug/ml

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aflatoxin B1

Aflatoxins (AF) are a group of harmful fungal toxins commonly found as grains/groundnuts contaminants and the most abundant congeners of AF, aflatoxin B1 (AFB1) is the most toxic among AFs which is produced by Aspergillus flavus, A. parasiticus etc. Humans and domestic animals can be exposed to aflatoxins by foods and approximately 25% of the world's food supply is annually contaminated with mycotoxins indicating the widespread prevalence of this public health problem. AFB1, a potent hepatotoxin/hepatocarcinogen, undergoes metabolism catalyzed by mixed function oxygenases of hepatic cytochrome P-450 enzyme system to produce a highly reactive intermediate, aflatoxin B1-8,9 epoxide that binds to necleophilic sites in hepatocyte DNA to form a number of adducts. The reactive aflatoxin B1-8,9 epoxide attracts guanine residues in double stranded DNA and the adducts formation leads to initiation of hepatocarcinogenesis. ROS generated during aflatoxins metablosm are considered to be main culprit for hepatotoxicity. The AFB1-8,9-epoxide can further be converted to AFB1-8,9-diol that specifically binds to lysine in albumin and forms AFB1-lysine adducts (AFB-Lys), which has been validated as a biomarker of human aflatoxins exposure.

Alternate Names

AFB1, AFB1-AR1, Aldoketoreductase 7

Additional Aflatoxin B1 Products

Product Documents for Aflatoxin B1 Antibody (6A10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Aflatoxin B1 Antibody (6A10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.