AGAP1 Antibody (OTI4E8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01708

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4E8

Concentration

0.5 mg/ml

View all AGAP1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human AGAP1(NP_055729) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for AGAP1 Antibody (OTI4E8)

Western Blot: AGAP1 Antibody (OTI4E8) [NBP2-01708]

Western Blot: AGAP1 Antibody (OTI4E8) [NBP2-01708]

Western Blot: AGAP1 Antibody (OTI4E8) [NBP2-01708] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGAP1.
Immunohistochemistry-Paraffin: AGAP1 Antibody (OTI4E8) [NBP2-01708]

Immunohistochemistry-Paraffin: AGAP1 Antibody (OTI4E8) [NBP2-01708]

Immunohistochemistry-Paraffin: AGAP1 Antibody (OTI4E8) [NBP2-01708] - Staining of paraffin-embedded Human tonsil using anti-AGAP1 mouse monoclonal antibody.
Flow Cytometry: AGAP1 Antibody (OTI4E8) [NBP2-01708]

Flow Cytometry: AGAP1 Antibody (OTI4E8) [NBP2-01708]

Flow Cytometry: AGAP1 Antibody (OTI4E8) [NBP2-01708] - Analysis of Jurkat cells, using anti-AGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for AGAP1 Antibody (OTI4E8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AGAP1

CENTG2 belongs to an ADP-ribosylation factor GTPase-activating (ARF-GAP) protein family involved in membrane traffic and actin cytoskeleton dynamics (Nie et al., 2002 [PubMed 12388557]).[supplied by OMIM].

Alternate Names

AGAP-1, ANK repeat and PH domains 1, ArfGAP with GTPase domain, ankyrin repeat and PH domain 1, centaurin, gamma 2, Centaurin-gamma-2, CENTG2, cnt-g2, GTP-binding and GTPase-activating protein 1, KIAA1099arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1, MGC71657

Entrez Gene IDs

116987 (Human)

Gene Symbol

AGAP1

Additional AGAP1 Products

Product Documents for AGAP1 Antibody (OTI4E8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AGAP1 Antibody (OTI4E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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