AGAP1 Antibody (OTI4E8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01708
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4E8
Concentration
0.5 mg/ml
Product Summary for AGAP1 Antibody (OTI4E8)
Immunogen
Full length human recombinant protein of human AGAP1(NP_055729) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for AGAP1 Antibody (OTI4E8)
Western Blot: AGAP1 Antibody (OTI4E8) [NBP2-01708]
Western Blot: AGAP1 Antibody (OTI4E8) [NBP2-01708] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGAP1.Immunohistochemistry-Paraffin: AGAP1 Antibody (OTI4E8) [NBP2-01708]
Immunohistochemistry-Paraffin: AGAP1 Antibody (OTI4E8) [NBP2-01708] - Staining of paraffin-embedded Human tonsil using anti-AGAP1 mouse monoclonal antibody.Flow Cytometry: AGAP1 Antibody (OTI4E8) [NBP2-01708]
Flow Cytometry: AGAP1 Antibody (OTI4E8) [NBP2-01708] - Analysis of Jurkat cells, using anti-AGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).Applications for AGAP1 Antibody (OTI4E8)
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AGAP1
Alternate Names
AGAP-1, ANK repeat and PH domains 1, ArfGAP with GTPase domain, ankyrin repeat and PH domain 1, centaurin, gamma 2, Centaurin-gamma-2, CENTG2, cnt-g2, GTP-binding and GTPase-activating protein 1, KIAA1099arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1, MGC71657
Entrez Gene IDs
116987 (Human)
Gene Symbol
AGAP1
Additional AGAP1 Products
Product Documents for AGAP1 Antibody (OTI4E8)
Product Specific Notices for AGAP1 Antibody (OTI4E8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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