AGAP1 Antibody (OTI4E8) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72357
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4E8
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human AGAP1(NP_055729) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for AGAP1 Antibody (OTI4E8) - Azide and BSA Free
Immunohistochemistry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]
Immunohistochemistry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357] - Staining of paraffin-embedded Human tonsil using anti-AGAP1 mouse monoclonal antibody.Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]
Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357] - Analysis of Jurkat cells, using anti-AGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357]
Flow Cytometry: AGAP1 Antibody (OTI4E8) - Azide and BSA Free [NBP2-72357] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGAP1.Applications for AGAP1 Antibody (OTI4E8) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AGAP1
Alternate Names
AGAP-1, ANK repeat and PH domains 1, ArfGAP with GTPase domain, ankyrin repeat and PH domain 1, centaurin, gamma 2, Centaurin-gamma-2, CENTG2, cnt-g2, GTP-binding and GTPase-activating protein 1, KIAA1099arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1, MGC71657
Gene Symbol
AGAP1
Additional AGAP1 Products
Product Documents for AGAP1 Antibody (OTI4E8) - Azide and BSA Free
Product Specific Notices for AGAP1 Antibody (OTI4E8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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