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AGAP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1323

Novus Biologicals, part of Bio-Techne
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NB100-1323

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-NPSAELHRSPSLL corresponding to C-Terminus according to NP_114152.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for AGAP3 Antibody

Immunohistochemistry-Paraffin: AGAP3 Antibody [NB100-1323]

Immunohistochemistry-Paraffin: AGAP3 Antibody [NB100-1323]

Immunohistochemistry-Paraffin: AGAP3 Antibody [NB100-1323] - (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for AGAP3 Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Immunohistochemistry-Paraffin

5 - 10 ug/ml

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 45 kDa band in human brain and human liver lysates at 1 ug/ml, this band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in scientific literature for the band we observe given the predicted MW of approx. 101 kDa according to NP_114152. IHC-P: Human kidney shows strong staining of brush borders in proximal convoluted tubules.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AGAP3

CENTG3, or M-RIP, is a 116 kDa protein that interacts with myosin phosphatase and RhoA in vascular smooth muscle cells. Because CENTG3 is able to assemble a complex containing both RhoA and MBS, it is speculated that this protein may play a role in myosin phosphatase regulation.

Alternate Names

ANK repeat and PH domain-containing protein 3, ArfGAP with GTPase domain, ankyrin repeat and PH domain 3, centaurin-gamma-3, cnt-g3, CRAG, CRAM-associated GTPase, CRMP (collapsin response mediator protein) associated, FLJ16146, FLJ34452, gamma 3, MR1-interacting protein, MRIP-1

Entrez Gene IDs

116988 (Human); 213990 (Mouse)

Gene Symbol

AGAP3

Additional AGAP3 Products

Product Documents for AGAP3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AGAP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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