AGER Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13484
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Amphibian, Squirrel
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for AGER Antibody
Immunogen
Synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat AGER.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for AGER Antibody
Western Blot: AGER Antibody [NBP3-13484]
Western Blot: AGER Antibody [NBP3-13484] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AGER antibody (NBP3-13484) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunohistochemistry-Paraffin: AGER Antibody [NBP3-13484]
Immunohistochemistry-Paraffin: AGER Antibody [NBP3-13484] - IHC-P analysis of normal mouse lymph node tissue with (ledt) or without (right) AGER antibody at a dilution of 1:20. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15minWestern Blot: AGER Antibody [NBP3-13484]
Western Blot: AGER Antibody [NBP3-13484] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with AGER antibody (NBP3-13484) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for AGER Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
1-2 ug/ml
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RAGE/AGER
Long Name
Receptor for Advanced Glycation End Products
Alternate Names
AGER, SCARJ1
Gene Symbol
AGER
Additional RAGE/AGER Products
Product Specific Notices for AGER Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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