Aggrecan Antibody (6-B-4)
Novus Biologicals, part of Bio-Techne | Catalog # NB110-6524
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Bovine
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 6-B-4
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Synthetic peptide: EPEEPFTFAPEI.
Reactivity Notes
Cross-reacts with Human and Cow. Sheep data from customer review. Mouse reactivity reported in scientific literature (PMID: 31364704).
Specificity
Recognises the linear amino acid sequence 394EPEEPFTFAPEI406 present in the interglobular domain (IGD) of human and bovine aggrecan.
Marker
Chondrocyte Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aggrecan Antibody (6-B-4)
![Immunohistochemistry-Paraffin: Aggrecan Antibody (6-B-4) [NB110-6524]](https://resources.bio-techne.com/images/products/Aggrecan-Antibody-6-B-4-Immunohistochemistry-Paraffin-NB110-6524-img0001.jpg "Immunohistochemistry-Paraffin: Aggrecan Antibody (6-B-4) [NB110-6524]")
Immunohistochemistry-Paraffin: Aggrecan Antibody (6-B-4) [NB110-6524]
Immunohistochemistry-Paraffin: Aggrecan Antibody (6-B-4) [NB110-6524] - Aggrecan on sheep intervertebral disc. Image from verified customer review.Applications for Aggrecan Antibody (6-B-4)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100
Application Notes
This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NB110-6524 in the following applications:
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
Tissue culture supernatant
Preservative
0.002% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aggrecan
Additional Aggrecan Products
Product Documents for Aggrecan Antibody (6-B-4)
Product Specific Notices for Aggrecan Antibody (6-B-4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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