Aggrecan Antibody (6L4T2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33191
Key Product Details
Species Reactivity
Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 6L4T2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 761-861 of human Aggrecan (P16112).
Sequence:
WPPTGAATEESTEGPSATEVPSASEEPSPSEVPFPSEEPSPSEEPFPSVRPFPSVELFPSEEPFPSKEPSPSEEPSASEEPYTPSPPVPSWTELPSSGEES
Sequence:
WPPTGAATEESTEGPSATEVPSASEEPSPSEVPFPSEEPSPSEEPFPSVRPFPSVELFPSEEPFPSKEPSPSEEPSASEEPYTPSPPVPSWTELPSSGEES
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
261 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aggrecan Antibody (6L4T2)
Immunohistochemistry: Aggrecan Antibody (6L4T2) [NBP3-33191] -
Immunohistochemistry: Aggrecan Antibody (6L4T2) [NBP3-33191] - Immunohistochemistry analysis of paraffin-embedded Mouse bone marrow using Aggrecan Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Western Blot: Aggrecan Antibody (6L4T2) [NBP3-33191] -
Western Blot: Aggrecan Antibody (6L4T2) [NBP3-33191] - Western blot analysis of lysates from Mouse lung, using Aggrecan Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry: Aggrecan Antibody (6L4T2) [NBP3-33191] -
Immunohistochemistry: Aggrecan Antibody (6L4T2) [NBP3-33191] - Immunohistochemistry analysis of paraffin-embedded Rat bone marrow using Aggrecan Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.Applications for Aggrecan Antibody (6L4T2)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Aggrecan
Alternate Names
ACAN, AGC1, CSPG1, CSPGCP, MSK16, SEDK
Gene Symbol
ACAN
Additional Aggrecan Products
Product Documents for Aggrecan Antibody (6L4T2)
Product Specific Notices for Aggrecan Antibody (6L4T2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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