Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Equine, Feline, Guinea Pig, Rabbit, Sheep

Cited:

Human, Mouse, Rat, Bovine, Rabbit

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # BC-3

Format

BSA Free

Concentration

1.0 mg/ml

View all Aggrecan Antibodies »

Product Specifications

Immunogen

ARGSV... synthetic peptide conjugate.

Reactivity Notes

Cross-reacts with Human, Cow, Cat, Dog, Guinea pig, Horse, Pig, Rabbit, Rat and Sheep. Mouse reactivity reported in scientific literature (PMID: 27185069). Not yet tested in other species.

Localization

Secreted

Specificity

Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan.

Marker

Chondrocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aggrecan Antibody (BC-3) - BSA Free

Western Blot: Aggrecan Antibody (BC-3)BSA Free [NB600-504]

Western Blot: Aggrecan Antibody (BC-3)BSA Free [NB600-504]

Western Blot: Aggrecan Antibody (BC-3) [NB600-504] - P-PRP induces more extracellular matrix-related proteins. Production of collagen II and aggrecan, as measured by western blot. Image collected and cropped by Citeab from the following publication (The Differential Effects of Leukocyte-Containing and Pure Platelet-Rich Plasma on Nucleus Pulposus-Derived Mesenchymal Stem Cells: Implications for the Clinical Treatment of Intervertebral Disc Degeneration. Stem Cells Int (2018)) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Aggrecan Antibody (BC-3) - BSA Free [NB600-504]

Immunocytochemistry/ Immunofluorescence: Aggrecan Antibody (BC-3) - BSA Free [NB600-504]

Immunocytochemistry/Immunofluorescence: Aggrecan Antibody (BC-3) [NB600-504] - P-PRP induces more extracellular matrix-related proteins. Collagen II and aggrecan in the cytoplasm of the coculture cells imaged by fluorescence microscopy. Image collected and cropped by Citeab from the following publication (The Differential Effects of Leukocyte-Containing and Pure Platelet-Rich Plasma on Nucleus Pulposus-Derived Mesenchymal Stem Cells: Implications for the Clinical Treatment of Intervertebral Disc Degeneration. Stem Cells Int (2018)) licensed under a CC-BY license.
Western Blot: Aggrecan Antibody (BC-3)BSA Free [NB600-504]

Western Blot: Aggrecan Antibody (BC-3)BSA Free [NB600-504]

Western Blot: Aggrecan Antibody (BC-3) [NB600-504] - Expression levels of related genes during differentiation induction in the RCCS environment. Expression of ACAN, collagen II, collagen X and Runx2 was detected by western blotting on day 10 during differentiation induction. Image collected and cropped by Citeab from the following publication (Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system. Cell Mol Biol Lett (2019)) licensed under a CC-BY license.
View 2 more images

Applications for Aggrecan Antibody (BC-3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100
Application Notes
This Aggrecan antibody is useful in Immunohistochemistry, ELISA and Western blot. With IHC the antibody works in formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections. In Western blot the antibody detects a variety of epitopes between 50 and 250 kDa. Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27185069).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aggrecan

The Aggrecan degradation product usually remains within the tissue still complexed to hyaluronan and link protein. Its release from the cartilage usually signals that there has been extensive catabolism of aggrecan, which allows large complexes containing this metabolite to be released from the tissue. Aggrecan degradation products can be found in the synovial fluids of patients with degenerative joint disease.

Alternate Names

ACAN, AGC1, CSPG1, CSPGCP, MSK16, SEDK

Entrez Gene IDs

176 (Human); 58968 (Rat)

Gene Symbol

ACAN

UniProt

P16112

Additional Aggrecan Products

Product Documents for Aggrecan Antibody (BC-3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Aggrecan Antibody (BC-3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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