Aggrecan Antibody (BC-3) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB600-504B
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Canine, Equine, Feline, Guinea Pig, Rabbit, Sheep
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BC-3
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Summary for Aggrecan Antibody (BC-3) [Biotin]
Immunogen
ARGSV... synthetic peptide conjugate.
Reactivity Notes
Cross-reacts with Human, Cow, Cat, Dog, Guinea pig, Horse, Pig, Rabbit, Rat and Sheep. Mouse reactivity reported in scientific literature (PMID: 27185069). Not yet tested in other species.
Localization
Secreted
Specificity
Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan.
Marker
Chondrocyte Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Applications for Aggrecan Antibody (BC-3) [Biotin]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Aggrecan antibody is useful in Immunohistochemistry, ELISA and Western blot. With IHC the antibody works in formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections. In Western blot the antibody detects a variety of epitopes between 50 and 250 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Aggrecan
Alternate Names
ACAN, AGC1, CSPG1, CSPGCP, MSK16, SEDK
Gene Symbol
ACAN
Additional Aggrecan Products
Product Documents for Aggrecan Antibody (BC-3) [Biotin]
Product Specific Notices for Aggrecan Antibody (BC-3) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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