Aggrecan Antibody (MK-302)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29670
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Canine, Rabbit, Sheep
Applications
ELISA, Radioimmunoassay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # MK-302
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Chondroitinase ABC-digested adult human aggrecan.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Native proteoglycans do not react with MAB2035. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. No cross-reactivity with dermatan sulfate, keratan sulfate or chondroitin-4-sulfate.
Marker
Chondrocyte Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Relevant Entrez Gene Numbers: NM_001135.2;NM_013227.2
Applications for Aggrecan Antibody (MK-302)
Application
Recommended Usage
ELISA
1:100-1:5000
Radioimmunoassay
1:100-1:200
Western Blot
1:100-1:2000
Application Notes
Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. {0.5U/mL in 100mM Tris-HCL, 30 minutes room temperature} A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Aggrecan
Additional Aggrecan Products
Product Documents for Aggrecan Antibody (MK-302)
Product Specific Notices for Aggrecan Antibody (MK-302)
Relevant Entrez Gene Numbers: NM_001135.2;NM_013227.2
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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