Aggrecan Neoepitope Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-74350
![Western Blot: Aggrecan Neoepitope AntibodyBSA Free [NB100-74350]](https://resources.bio-techne.com/images/products/Aggrecan-Neoepitope-Antibody-Western-Blot-NB100-74350-img0002.jpg "Western Blot: Aggrecan Neoepitope AntibodyBSA Free [NB100-74350]")
![Western Blot: Aggrecan Neoepitope AntibodyBSA Free [NB100-74350]](https://resources.bio-techne.com/images/products/Aggrecan-Neoepitope-Antibody-Western-Blot-NB100-74350-img0006.jpg "Western Blot: Aggrecan Neoepitope AntibodyBSA Free [NB100-74350]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Canine
Cited:
Human, Mouse, Rat, Porcine
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A synthetic peptide corresponding to a region of human Aggrecan (within residues 350-400). [UniProt# P16112]
Specificity
Aggrecan Neo (JSCNIT)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aggrecan Neoepitope Antibody - BSA Free
Western Blot: Aggrecan Neoepitope AntibodyBSA Free [NB100-74350]
Western Blot: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in L929 whole cell extract using NB100-74350.![Immunocytochemistry/ Immunofluorescence: Aggrecan Neoepitope Antibody - BSA Free [NB100-74350]](https://resources.bio-techne.com/images/products/Aggrecan-Neoepitope-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74350-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Aggrecan Neoepitope Antibody - BSA Free [NB100-74350]")
Immunocytochemistry/ Immunofluorescence: Aggrecan Neoepitope Antibody - BSA Free [NB100-74350]
Immunocytochemistry/Immunofluorescence: Aggrecan Neoepitope Antibody [NB100-74350] - Detection of ACAN in OA cartilage using NB100-74350.![Immunohistochemistry: Aggrecan Neoepitope Antibody - BSA Free [NB100-74350]](https://resources.bio-techne.com/images/products/Aggrecan-Neoepitope-Antibody-Immunohistochemistry-NB100-74350-img0008.jpg "Immunohistochemistry: Aggrecan Neoepitope Antibody - BSA Free [NB100-74350]")
Immunohistochemistry: Aggrecan Neoepitope Antibody - BSA Free [NB100-74350]
Immunohistochemistry: Aggrecan Neoepitope Antibody [NB100-74350] - Analysis of ACAN in mouse cartilage with hematoxylin counterstain.Applications for Aggrecan Neoepitope Antibody - BSA Free
Application
Recommended Usage
Flow Cytometry
reported in scientific literature (PMID 21482542)
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Immunohistochemistry
1:1000
Immunohistochemistry-Frozen
reported in scientific literature (PMID 21482542)
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:1000
Application Notes
In WB a band is detected at ~61 kDa representing the neoepitope sequence (NITEGE) generated by aggrecanase-mediated cleavage at Glu373-Ala374 of aggrecan core proteins.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 30% Glycerol
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Aggrecan Neoepitope
Alternate Names
AGC1SEDK, aggrecan, aggrecan core protein, Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan 1, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1aggrecan 1, CSPGCP, large aggregating proteoglycan, MSK16AGCAN
Gene Symbol
ACAN
UniProt
Additional Aggrecan Neoepitope Products
Product Documents for Aggrecan Neoepitope Antibody - BSA Free
Product Specific Notices for Aggrecan Neoepitope Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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