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Aggrecan Neoepitope Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74350V

Novus Biologicals, part of Bio-Techne
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NB100-74350V
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Aggrecan Neoepitope Antibody [DyLight 405]

Immunogen

A synthetic peptide corresponding to a region of human Aggrecan (within residues 350-400). [UniProt# P16112]

Specificity

Aggrecan Neo (JSCNIT)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Aggrecan Neoepitope Antibody [DyLight 405]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Aggrecan Neoepitope

Aggrecan Neoepitope (ACAN) is a proteoglycan belonging to aggrecan/versican proteoglycan family and is a major component of extracellular matrix of cartilagenous tissues. ACAN proteoglycan is mainly consists of chondroitin sulfate, however, it also contains keratan sulfate chains, N/O-linked oligosaccharides. Aggrecanase mediated cleavage results in ACAN fragments release from articular cartilage into synovial fluid during different stages of human osteoarthritis. ACAN is a secreted protein found in extracellular space/extracellular matrix and is more or less restricted to cartilages where it interact with FBLN1 as well as COM for serving the role of resisting compression in cartilage. ACAN binds to hyaluronic acid via an N-terminal globular region with high avidity and its expression occurs in chondrocytes throughout the developing skeleton. ACAN defects have been linked to spondyloepiphyseal dysplasia type Kimberley (SEDK), spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN), osteochondritis dissecans short stature and early-onset osteoarthritis (OD).

Alternate Names

AGC1SEDK, aggrecan, aggrecan core protein, Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan 1, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1aggrecan 1, CSPGCP, large aggregating proteoglycan, MSK16AGCAN

Gene Symbol

ACAN

Additional Aggrecan Neoepitope Products

Product Documents for Aggrecan Neoepitope Antibody [DyLight 405]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aggrecan Neoepitope Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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