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AgRP/ART Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1540

Novus Biologicals, part of Bio-Techne
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NB100-1540

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence CRKLGTAMNPCSRT corresponding to C-Terminus according to NP_001129.1, NP_015531.1.

Epitope

C Terminus

Specificity

This antibody is expected to recognise both reported isoforms (as represented by NP_001129; NP_015531)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for AgRP/ART Antibody

Immunohistochemistry: AgRP/ART Antibody [NB100-1540]

Immunohistochemistry: AgRP/ART Antibody [NB100-1540]

Immunohistochemistry: AgRP/ART Antibody [NB100-1540] - Staining of PFA-perfused cryosection of Human Hypothalamus. Antibody at 0.05 ug/mL. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.

Applications for AgRP/ART Antibody

Application
Recommended Usage

Immunohistochemistry

0.05 - 0.1 ug/mL

Immunohistochemistry-Frozen

0.05 - 0.1 ug/mL

Peptide ELISA

Detection limit 1:32000
Application Notes
IHC-Fr: Human hypothalamus shows staining of a group of neuronal cell bodies in the human infundibular (arcuate) nucleus and their varicose axons.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AgRP/ART

AGRP is the endogenous antagonist of alpha-melanocyte stimulating hormone and has been shown to cause potent stimulation of food intake, and this protein is found in over 90% of Neuropeptide Y containing cells in rats.

Long Name

Agouti-related Protein

Alternate Names

ART

Entrez Gene IDs

181 (Human); 11604 (Mouse)

Gene Symbol

AGRP

Additional AgRP/ART Products

Product Documents for AgRP/ART Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AgRP/ART Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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