AHR Antibody (3B12)

Novus Biologicals, part of Bio-Techne | Catalog # H00000196-M02

Novus Biologicals, part of Bio-Techne
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H00000196-M02
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Chemotaxis, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AHR Antibodies »

Product Specifications

Immunogen

AHR (NP_001612, 721 a.a. ~ 820 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGSFEPSPYPTTSSLEDFVTCLQLPENQKHGLNPQSAIITPQTCYAGAVSMYQCQPEPQHTHVGQMQYNPVLPGQQAFLNKFQNGVLNETYPAELNNINN

Specificity

aryl hydrocarbon receptor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for AHR Antibody (3B12)

Western Blot: AHR Antibody (3B12) [H00000196-M02]

Western Blot: AHR Antibody (3B12) [H00000196-M02]

Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M02), clone 3B12.Lane 1: AHR transfected lysate(96.147 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: AHR Antibody (3B12) [H00000196-M02]

Immunocytochemistry/ Immunofluorescence: AHR Antibody (3B12) [H00000196-M02]

Immunocytochemistry/Immunofluorescence: AHR Antibody (3B12) [H00000196-M02] - Analysis of monoclonal antibody to AHR on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: AHR Antibody (3B12) [H00000196-M02]

Western Blot: AHR Antibody (3B12) [H00000196-M02]

Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in COLO 320 HSR ( Cat # L020V1 ).
View 3 more images

Applications for AHR Antibody (3B12)

Application
Recommended Usage

ELISA

0.03ng/ml

Immunocytochemistry/ Immunofluorescence

20ug/mL

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IF, RNAi Validation, and ELISA. ChIP usage reported in scientific literature (PMID: 23714156).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AHR

This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons.

Long Name

Aryl Hydrocarbon Receptor

Alternate Names

BHLHE76

Entrez Gene IDs

196 (Human)

Gene Symbol

AHR

OMIM

600253 (Human)

UniProt

NP_001612 (Human)

Additional AHR Products

Product Documents for AHR Antibody (3B12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for AHR Antibody (3B12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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