AHR Antibody (3B12)
Novus Biologicals, part of Bio-Techne | Catalog # H00000196-M02
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot
Cited:
Chemotaxis, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3B12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
AHR (NP_001612, 721 a.a. ~ 820 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGSFEPSPYPTTSSLEDFVTCLQLPENQKHGLNPQSAIITPQTCYAGAVSMYQCQPEPQHTHVGQMQYNPVLPGQQAFLNKFQNGVLNETYPAELNNINN
Specificity
aryl hydrocarbon receptor
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for AHR Antibody (3B12)
Western Blot: AHR Antibody (3B12) [H00000196-M02]
Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M02), clone 3B12.Lane 1: AHR transfected lysate(96.147 KDa).Lane 2: Non-transfected lysate.Immunocytochemistry/ Immunofluorescence: AHR Antibody (3B12) [H00000196-M02]
Immunocytochemistry/Immunofluorescence: AHR Antibody (3B12) [H00000196-M02] - Analysis of monoclonal antibody to AHR on HeLa cell. Antibody concentration 10 ug/ml.Western Blot: AHR Antibody (3B12) [H00000196-M02]
Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in COLO 320 HSR ( Cat # L020V1 ).Applications for AHR Antibody (3B12)
Application
Recommended Usage
ELISA
0.03ng/ml
Immunocytochemistry/ Immunofluorescence
20ug/mL
Immunoprecipitation
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IF, RNAi Validation, and ELISA. ChIP usage reported in scientific literature (PMID: 23714156).
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: AHR
Long Name
Aryl Hydrocarbon Receptor
Alternate Names
BHLHE76
Entrez Gene IDs
196 (Human)
Gene Symbol
AHR
OMIM
600253 (Human)
UniProt
Additional AHR Products
Product Documents for AHR Antibody (3B12)
Product Specific Notices for AHR Antibody (3B12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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