AHR Antibody (RPT9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB300-515

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # RPT9

Format

BSA Free

Concentration

1 mg/ml

View all AHR Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Detects the Aryl Hydrocarbon Receptor (AHR).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Scientific Data Images for AHR Antibody (RPT9) - BSA Free

Western Blot: AHR Antibody (RPT9)BSA Free [NB300-515]

Western Blot: AHR Antibody (RPT9)BSA Free [NB300-515]

Western Blot: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor was performed by loading 20 ug of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (right lane B) or empty vector control (left lane A) and 10ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel.
Immunocytochemistry/ Immunofluorescence: AHR Antibody (RPT9) - BSA Free [NB300-515]

Immunocytochemistry/ Immunofluorescence: AHR Antibody (RPT9) - BSA Free [NB300-515]

Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) shows staining in U251 Cells. Aryl Hydrocarbon Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Aryl Hydrocarbon Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Flow Cytometry: AHR Antibody (RPT9) - BSA Free [NB300-515]

Flow Cytometry: AHR Antibody (RPT9) - BSA Free [NB300-515]

Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in NIH/3T3 cells compared to an isotype control (blue).
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Applications for AHR Antibody (RPT9) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:100

ELISA

1:100 - 1:2000

Flow Cytometry

1:50

Gel Super Shift Assays

1:1 - 1:100

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500 - 1:2000
Application Notes
ELISA use reported in the literature (PMID: 10413464). IP: A single ~95 kDa protein representing AHR from Hepa 1 cytosol. IHC: Staining of AHR in rat liver results in strong cytoplasmic and some nuclear staining.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AHR

The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor. Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.

Long Name

Aryl Hydrocarbon Receptor

Alternate Names

BHLHE76

Entrez Gene IDs

196 (Human); 11622 (Mouse); 25690 (Rat)

Gene Symbol

AHR

UniProt

P30561 (Mouse)

Additional AHR Products

Product Documents for AHR Antibody (RPT9) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AHR Antibody (RPT9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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