AIDA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88323

Novus Biologicals, part of Bio-Techne
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NBP1-88323
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AIDA Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EEENLEFEEDEEEGGAGAGSPDSFPARVPGTLLPRLPSEPGMTLLTIRIEKIGLKDAGQCIDPYITVS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AIDA Antibody

Western Blot: AIDA Antibody [NBP1-88323]

Western Blot: AIDA Antibody [NBP1-88323]

Western Blot: AIDA Antibody [NBP1-88323] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: AIDA Antibody [NBP1-88323]

Immunocytochemistry/ Immunofluorescence: AIDA Antibody [NBP1-88323]

Immunocytochemistry/Immunofluorescence: AIDA Antibody [NBP1-88323] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & microtubules.
Immunohistochemistry-Paraffin: AIDA Antibody [NBP1-88323]

Immunohistochemistry-Paraffin: AIDA Antibody [NBP1-88323]

Immunohistochemistry-Paraffin: AIDA Antibody [NBP1-88323] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

Applications for AIDA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AIDA

C1orf80 acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin anddisrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathwayactivated by AXIN/JNK-signaling

Alternate Names

axin interactor, dorsalization associated, chromosome 1 open reading frame 80, dorsalization-associated protein, FLJ12806, FLJ32421

Entrez Gene IDs

64853 (Human)

Gene Symbol

AIDA

UniProt

Q96BJ3 (Human)

Additional AIDA Products

Product Documents for AIDA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for AIDA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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