AIF Antibody (4B2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26515
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4B2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human AIF [UniProt O95831]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AIF Antibody (4B2)
Immunohistochemistry: AIF Antibody (4B2) [NBP3-26515] -
Immunohistochemistry: AIF Antibody (4B2) [NBP3-26515] - Image of AIF Antibody (4B2) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: AIF Antibody (4B2) [NBP3-26515] -
Immunohistochemistry: AIF Antibody (4B2) [NBP3-26515] - Image of AIF Antibody (4B2) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: AIF Antibody (4B2) [NBP3-26515] -
Western Blot: AIF Antibody (4B2) [NBP3-26515] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Rat liver tissue, Rat kidney tissue.All lanes: AIF Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 67, 36, 29, 27 kDa
Observed band size: 67 kDa
Applications for AIF Antibody (4B2)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: AIF
Long Name
Apoptosis Inducing Factor
Alternate Names
AIFM1, COXPD6, PDCD8
Gene Symbol
AIFM1
Additional AIF Products
Product Documents for AIF Antibody (4B2)
Product Specific Notices for AIF Antibody (4B2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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