AK5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89453

Novus Biologicals, part of Bio-Techne
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NBP1-89453
NBP1-89453-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AK5 Antibodies »

Product Summary for AK5 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KLFPNKEAAAGSSDLDPSMILDTGEIIDTGSDYEDQGDDQLNVFGEDTMGGFMEDLRKCKIIFIIGGPGSGKGTQCEKLVE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AK5 Antibody

Western Blot: AK5 Antibody [NBP1-89453]

Western Blot: AK5 Antibody [NBP1-89453]

Western Blot: AK5 Antibody [NBP1-89453] - Analysis in control (vector only transfected HEK293T lysate) and AK5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: AK5 Antibody [NBP1-89453]

Immunocytochemistry/ Immunofluorescence: AK5 Antibody [NBP1-89453]

Immunocytochemistry/Immunofluorescence: AK5 Antibody [NBP1-89453] - Staining of human cell line LHCN-M2 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: AK5 Antibody [NBP1-89453]

Immunohistochemistry-Paraffin: AK5 Antibody [NBP1-89453]

Immunohistochemistry-Paraffin: AK5 Antibody [NBP1-89453] - Staining in human cerebral cortex and liver tissues using anti-AK5 antibody. Corresponding AK5 RNA-seq data are presented for the same tissues.
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Applications for AK5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AK5

AK5 encodes a member of the adenylate kinase family, which is involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. This member is related to the UMP/CMP kinase of several species. It is located in the cytosol and expressed exclusively in brain. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.

Long Name

Adenylate kinase isoenzyme 5

Alternate Names

AK 5

Entrez Gene IDs

26289 (Human)

Gene Symbol

AK5

UniProt

Q9Y6K8 (Human)

Additional AK5 Products

Product Documents for AK5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AK5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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