AKAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89172

Novus Biologicals, part of Bio-Techne
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NBP1-89172
NBP1-89172-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AKAP1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EHVLELENSKGPSLASLEGEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELAKDDAAPAPPVADAKAQDRGVEGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQIISQVISEATEQV

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23172368).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AKAP1 Antibody

Western Blot: AKAP1 Antibody [NBP1-89172]

Western Blot: AKAP1 Antibody [NBP1-89172]

Western Blot: AKAP1 Antibody [NBP1-89172] - Analysis in control (vector only transfected HEK293T lysate) and AKAP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: AKAP1 Antibody [NBP1-89172]

Immunohistochemistry-Paraffin: AKAP1 Antibody [NBP1-89172]

Immunohistochemistry-Paraffin: AKAP1 Antibody [NBP1-89172] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: AKAP1 Antibody [NBP1-89172]

Immunohistochemistry-Paraffin: AKAP1 Antibody [NBP1-89172]

Immunohistochemistry-Paraffin: AKAP1 Antibody [NBP1-89172] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
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Applications for AKAP1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AKAP1

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.

Alternate Names

A kinase (PRKA) anchor protein 1, a kinase anchor protein 1, mitochondrial, AKAP121, AKAP149AKAP 149, AKAP84, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa, A-kinase anchor protein, 149kD, D-AKAP1, D-AKAP-1, Dual specificity A-kinase-anchoring protein 1, dual-specificity A-kinase anchoring protein 1, MGC1807, PRKA1, protein kinase A anchoring protein 1, protein kinase A1, Protein kinase A-anchoring protein 1, protein kinase anchoring protein 1, S-AKAP84, SAKAP84AKAP, Spermatid A-kinase anchor protein 84

Entrez Gene IDs

8165 (Human)

Gene Symbol

AKAP1

UniProt

Q92667 (Human)

Additional AKAP1 Products

Product Documents for AKAP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AKAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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