AKR1A1 Antibody (OTI6E3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71591

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI6E3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all AKR1A1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human AKR1A1 (NP_006057) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

36.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AKR1A1 Antibody (OTI6E3) - Azide and BSA Free

Western Blot: AKR1A1 Antibody (OTI6E3)Azide and BSA Free [NBP2-71591]

Western Blot: AKR1A1 Antibody (OTI6E3)Azide and BSA Free [NBP2-71591]

Western Blot: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.
Immunocytochemistry/ Immunofluorescence: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591]

Immunocytochemistry/ Immunofluorescence: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591]

Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKR1A1.
Immunohistochemistry: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591]

Immunohistochemistry: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591]

Immunohistochemistry: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - Staining of paraffin-embedded liver tissue using anti-AKR1A1 mouse monoclonal antibody.
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Applications for AKR1A1 Antibody (OTI6E3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AKR1A1

AKR1A1 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Alternative splicing of this gene results in two transcript variants encoding the same protein.

Long Name

Aldo-keto reductase family 1 member A1

Alternate Names

Aldehyde reductase, ALDR1, EC 1.1.1.19, EC 1.1.1.2, EC 1.1.1.20, EC 1.1.1.372, EC 1.1.1.54

Gene Symbol

AKR1A1

Additional AKR1A1 Products

Product Documents for AKR1A1 Antibody (OTI6E3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AKR1A1 Antibody (OTI6E3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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