AKT1/2/3 Antibody (SR2006)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21678

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21678-100ul
NBP3-21678-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2006

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AKT1/2/3 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human AKT1/2/3 (Uniprot #: P31749, P31751, Q9Y243)

Specificity

Detects endogenous levels of total AKT1/2/3

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AKT1/2/3 Antibody (SR2006)

Immunohistochemistry: AKT1/2/3 Antibody (SR2006) [NBP3-21678] -

Immunohistochemistry: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunohistochemical analysis of paraffin-embedded human testis, using NBP3-21678.

Immunocytochemistry/Immunofluorescence: AKT1/2/3 Antibody (SR2006) [NBP3-21678] -

Immunocytochemistry/Immunofluorescence: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunofluorescent analysis of MCF-7 cells, using NBP3-21678.

Western Blot: AKT1/2/3 Antibody (SR2006) [NBP3-21678] -

Western Blot: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Western blot analysis using NBP3-21678 in (1) A549 cell lysate; (2) Mouse brain lysate; (3) Rat brain lysate; (4) Rat heart lysate; (5) Rat kidney lysate.

Applications for AKT1/2/3 Antibody (SR2006)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AKT1/2/3

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. AKT has been shown to play a role in such metabolic processes as glucose transport, glycogen synthesis, glycolysis, and protein synthesis. It had also been shown to promote cell survival by inhibiting apoptosis through its ability to phosphoylate and inactivate several targets, including Bad, Forkhead transcription factors, and caspase 9. Activity of AKT has been associated with the phosphorylation of two sites: T308, in the activation loop of the kinase, and S473, at the carboxyl terminus. Phosphorylation of both sites contributes to AKT activity, however phosphorylation of T308 has been shown to be absolutely essential for AKT activation.

Alternate Names

AKT, EC 2.7.11, EC 2.7.11.1, PKBMGC99656, PRKBA, Protein kinase B, Proto-oncogene c-Akt, rac protein kinase alpha, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, RACPKB-ALPHA, v-akt murine thymoma viral oncogene homolog 1

Gene Symbol

AKT1

Additional AKT1/2/3 Products

Product Documents for AKT1/2/3 Antibody (SR2006)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AKT1/2/3 Antibody (SR2006)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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