Albumin Antibody (ALB/6411R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07174
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Albumin Antibody (ALB/6411R) [NBP3-07174]](https://resources.bio-techne.com/images/products/Albumin-Antibody-ALB-6411R-Immunohistochemistry-Paraffin-NBP3-07174-img0001.jpg "Immunohistochemistry-Paraffin: Albumin Antibody (ALB/6411R) [NBP3-07174]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # ALB/6411R
Concentration
0.2 mg/ml
Product Summary for Albumin Antibody (ALB/6411R)
Immunogen
Recombinant full-length human Albumin protein (Uniprot: P02768 )
Localization
Cell surface, Cytoplasm
Specificity
This monoclonal antibody is absolutely specific to albumin and does not show any significant cross-reaction with other human proteins. Albumin is a soluble, monomeric protein, which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted form of albumin.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08917)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Albumin Antibody (ALB/6411R)
Immunohistochemistry-Paraffin: Albumin Antibody (ALB/6411R) [NBP3-07174]
Immunohistochemistry-Paraffin: Albumin Antibody (ALB/6411R) [NBP3-07174] - Formalin-fixed, paraffin-embedded human liver carcinoma in colon stained with Albumin Recombinant Rabbit Monoclonal Antibody (ALB/6411R).Applications for Albumin Antibody (ALB/6411R)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Albumin
Additional Albumin Products
Product Documents for Albumin Antibody (ALB/6411R)
Product Specific Notices for Albumin Antibody (ALB/6411R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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