ALCAM/CD166 Antibody (21B9) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26512
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 21B9
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human ALCAM/CD166 [UniProt Q13740]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ALCAM/CD166 Antibody (21B9) - BSA Free
Immunohistochemistry: ALCAM/CD166 Antibody (21B9) [NBP3-26512] -
Immunohistochemistry: ALCAM/CD166 Antibody (21B9) [NBP3-26512] - Image of ALCAM/CD166 Antibody (21B9) diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: ALCAM/CD166 Antibody (21B9) [NBP3-26512] -
Immunohistochemistry: ALCAM/CD166 Antibody (21B9) [NBP3-26512] - Image of ALCAM/CD166 Antibody (21B9) diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot: ALCAM/CD166 Antibody (21B9) [NBP3-26512] -
Western Blot: ALCAM/CD166 Antibody (21B9) [NBP3-26512] - Positive Western Blot detected in: PC3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate, Mouse brain tissue.All lanes: ALCAM/CD166 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 66, 64, 16, 34 kDa
Observed band size: 100 kDa
Applications for ALCAM/CD166 Antibody (21B9) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: ALCAM/CD166
Long Name
Activated Leukocyte Cell Adhesion Molecule
Alternate Names
CD166
Gene Symbol
ALCAM
Additional ALCAM/CD166 Products
Product Documents for ALCAM/CD166 Antibody (21B9) - BSA Free
Product Specific Notices for ALCAM/CD166 Antibody (21B9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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