alcohol dehydrogenase 7 Antibody (OTI2H10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03741

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2H10

Concentration

1 mg/ml

View all alcohol dehydrogenase 7 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ADH7(NP_000664) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

41.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alcohol dehydrogenase 7 Antibody (OTI2H10)

Western Blot: alcohol dehydrogenase 7 Antibody (OTI2H10) [NBP2-03741]

Western Blot: alcohol dehydrogenase 7 Antibody (OTI2H10) [NBP2-03741]

Western Blot: alcohol dehydrogenase 7 Antibody (2H10) [NBP2-03741] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY alcohol dehydrogenase 7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-alcohol dehydrogenase 7.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 7 Antibody (2H10) [NBP2-03741] Staining of paraffin-embedded Human tonsil using anti-alcohol dehydrogenase 7 mouse monoclonal antibody.
Flow Cytometry: alcohol dehydrogenase 7 Antibody (OTI2H10) [NBP2-03741]

Flow Cytometry: alcohol dehydrogenase 7 Antibody (OTI2H10) [NBP2-03741]

Flow Cytometry: alcohol dehydrogenase 7 Antibody (2H10) [NBP2-03741] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-alcohol dehydrogenase 7 antibody, and then analyzed by flow cytometry.
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Applications for alcohol dehydrogenase 7 Antibody (OTI2H10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alcohol dehydrogenase 7

This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members. Alternative splicing results in multiple transcript variants. [provided by RefSeq].

Alternate Names

ADH4, ADH-4, alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide, alcohol dehydrogenase class 4 mu/sigma chain, Alcohol dehydrogenase class IV mu/sigma chain, alcohol dehydrogenase VII, alcohol dehydrogenase-7, class IV sigma-1 alcohol dehydrogenase, class IV sigmasigma alcohol dehydrogenase, EC 1.1.1, EC 1.1.1.1, Gastric alcohol dehydrogenase, Retinol dehydrogenase

Entrez Gene IDs

131 (Human)

Gene Symbol

ADH7

Additional alcohol dehydrogenase 7 Products

Product Documents for alcohol dehydrogenase 7 Antibody (OTI2H10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alcohol dehydrogenase 7 Antibody (OTI2H10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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