Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70152

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1B6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ALDH3A1(NP_000682) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

50.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6)Azide and BSA Free [NBP2-70152]

Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6)Azide and BSA Free [NBP2-70152]

Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-70152] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH3A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH3A1.
Immunocytochemistry/ Immunofluorescence: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-70152]

Immunocytochemistry/ Immunofluorescence: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-70152]

Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-70152] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH3A1.
Immunohistochemistry: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-70152]

Immunohistochemistry: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-70152]

Immunohistochemistry: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-70152] - Staining of paraffin-embedded pancreas tissue using anti-ALDH3A1 mouse monoclonal antibody.
View 6 more images

Applications

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aldehyde Dehydrogenase 3-A1/ALDH3A1

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Alternate Names

Aldehyde Dehydrogenase 3A1, ALDH3A1, ALDHIII

Gene Symbol

ALDH3A1

Additional Aldehyde Dehydrogenase 3-A1/ALDH3A1 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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