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Aldehyde dehydrogenase 5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90192

Novus Biologicals, part of Bio-Techne
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NBP1-90192
NBP1-90192-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MLRFLAPRLLSLQGRTARYSSAAALPSPILNPDIPYNQLFINNEWQDAVSKKTFPTVNPTTGEVIGHV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aldehyde dehydrogenase 5 Antibody

Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-90192]

Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-90192]

Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Analysis in human liver and tonsil tissues using NBP1-90192 antibody. Corresponding ALDH1B1 RNA-seq data are presented for the same tissues.
Western Blot: Aldehyde dehydrogenase 5 Antibody [NBP1-90192]

Western Blot: Aldehyde dehydrogenase 5 Antibody [NBP1-90192]

Western Blot: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Analysis in human cell line RH-30.
Immunocytochemistry/ Immunofluorescence: Aldehyde dehydrogenase 5 Antibody [NBP1-90192]

Immunocytochemistry/ Immunofluorescence: Aldehyde dehydrogenase 5 Antibody [NBP1-90192]

Immunocytochemistry/Immunofluorescence: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.

Applications for Aldehyde dehydrogenase 5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 µg/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aldehyde dehydrogenase 5

Aldehyde dehydrogenase 5 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. This gene does not contain introns in the coding sequence. The variation of this locus may affect the development of alcohol-related problems. [provided by RefSeq]

Alternate Names

aldehyde dehydrogenase 1 family, member B1, Aldehyde dehydrogenase 5, Aldehyde dehydrogenase family 1 member B1, ALDH class 2, ALDH5aldehyde dehydrogenase X, mitochondrial, ALDHXacetaldehyde dehydrogenase 5, EC 1.2.1, EC 1.2.1.3, MGC2230, mitochondrial aldehyde dehydrogenase X

Gene Symbol

ALDH1B1

Additional Aldehyde dehydrogenase 5 Products

Product Documents for Aldehyde dehydrogenase 5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aldehyde dehydrogenase 5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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