Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry Free-Floating

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2E7

Concentration

1 mg/ml

View all ALDH1L1 Antibodies »

Product Specifications

Immunogen

Amino acids 402-902 of human ALDH1L1. [UniProt# O75891]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALDH1L1 Antibody (2E7)

Western Blot: ALDH1L1 Antibody (2E7) [NBP2-50033]

Western Blot: ALDH1L1 Antibody (2E7) [NBP2-50033]

Western Blot: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of different tissue and cell lysates using mouse mAb to ALDH1L1, NBP2-50033, dilution 1:5,000 in green: [1] protein standard (red), rat tissue lysates: [2] heart, [3] liver, [4] kidney,[5] lung, [6] brain, and [7] spinal cord; mouse tissue lysates: [8] brain, and [9] spinal cord; cell lysates: [10] NIH-3T3, and [11] HEK293. The band at 100kDa mark corresponds to ALDH1L1 protein.
Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033]

Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033]

Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to aldehyde dehydrogenase 1L1 (ALDH1L1), NBP2-50033, dilution 1:1,000 in red, and costained with chicken pAb to GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. NBP2-50033 antibody produces cytoplasmic staining of glial cells, while the GFAP antibody labels the intermediate filament cytoskeleton in astrocytes and other glial cells. Some astrocytic cells express both ALDH1L1 and GFAP and appear yellow.
Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033]

Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033]

Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033] - Neuron-glia cell mixed cultures stained with NBP2-50033 (red) and chicken polyclonal antibody against Vimentin (NB300-223, green). Blue is a DNA stain. NBP2-50033 stains astrocytic cell bodies and processes. The fibroblastic cells contain only Vimentin and so are green, while astrocytes contain either Vimentin and ALDH1L1, so appearing golden, or predominantly ALDH1L1, in which case they appear red.

Applications for ALDH1L1 Antibody (2E7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:5000 - 1:10000

Reviewed Applications

Read 1 review rated 3 using NBP2-50033 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH1L1

ALDH1L1 is encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.

Alternate Names

10-formyltetrahydrofolate dehydrogenase, aldehyde dehydrogenase 1 family, member L1, aldehyde dehydrogenase family 1 member L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, DKFZp781N0997, EC 1.5.1.6,10-FTHFDH, FDH, formyltetrahydrofolate dehydrogenase, FTHFD10-fTHF

Gene Symbol

ALDH1L1

UniProt

O75891 (Human)

Additional ALDH1L1 Products

Product Documents for ALDH1L1 Antibody (2E7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ALDH1L1 Antibody (2E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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