Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 4A12

Concentration

1 mg/ml

View all ALDH1L1 Antibodies »

Product Specifications

Immunogen

Amino acids 1- 401 of human ALDH1L1. [UniProt# O75891]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALDH1L1 Antibody (4A12)

Western Blot: ALDH1L1 Antibody (4A12) [NBP2-50045]

Western Blot: ALDH1L1 Antibody (4A12) [NBP2-50045]

Western Blot: ALDH1L1 Antibody (4A12) [NBP2-50045] - Analysis of rat and mouse tissue lysates using mouse ALDH1L1 mAb, dilution 1:5000 (Green): [1] protein standard (Red), rat tissue lysates: [2] heart, [3] liver, [4] kidney, [5] lung, [6] brain, and [7] spinal cord; Also mouse tissue lysates: [8] brain, and [9] spinal cord. The band at 100kDa corresponds to ALDH1L1 protein. In line with published work, ALDH1L1 is a major protein of liver and CNS tissue.
Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (4A12) [NBP2-50045]

Immunocytochemistry/ Immunofluorescence: ALDH1L1 Antibody (4A12) [NBP2-50045]

Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (4A12) [NBP2-50045] - Analysis of mixed cortical neuron-glial cell culture from an E20 rat stained with mouse ALDH1L1 mAb, dilution 1:1,000 (Green), and costained with chicken Vimentin pAb, dilution 1:5000 (Red). DAPI staining of nuclear DNA (Blue). The ALDH1L1 antibody produces strong cytoplasmic staining of astrocytic glial cells, while the Vimentin antibody labels the intermediate filament cytoskeleton in fibroblasts and other glial cells. Some cells, presumably developing astrocytes, are stained by both ALDH1L1 and vimentin and so appear yellow.

Applications for ALDH1L1 Antibody (4A12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000-1:2000

Immunohistochemistry

1:1000-1:2000

Western Blot

1:5000-1:10000

Reviewed Applications

Read 1 review rated 4 using NBP2-50045 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH1L1

ALDH1L1 is encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.

Alternate Names

10-formyltetrahydrofolate dehydrogenase, aldehyde dehydrogenase 1 family, member L1, aldehyde dehydrogenase family 1 member L1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, DKFZp781N0997, EC 1.5.1.6,10-FTHFDH, FDH, formyltetrahydrofolate dehydrogenase, FTHFD10-fTHF

Gene Symbol

ALDH1L1

UniProt

O75891 (Human)

Additional ALDH1L1 Products

Product Documents for ALDH1L1 Antibody (4A12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ALDH1L1 Antibody (4A12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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