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ALDH2 Antibody (4G6A3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37397

Novus Biologicals, part of Bio-Techne
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NBP2-37397
NBP2-37397-0.025ml

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4G6A3

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Purified recombinant fragment of human ALDH2 (AA: 317-517) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALDH2 Antibody (4G6A3) - BSA Free

Western Blot: ALDH2 Antibody (4G6A3)BSA Free [NBP2-37397]

Western Blot: ALDH2 Antibody (4G6A3)BSA Free [NBP2-37397]

Western Blot: ALDH2 Antibody (4G6A3) [NBP2-37397] - Western blot analysis using ALDH2 mouse mAb against HepG2 (1), A549 (2) cell lysate, and Rat live (3), Mouse liver (4), Mouse brain (5) tissue lysate.
Immunocytochemistry/ Immunofluorescence: ALDH2 Antibody (4G6A3) - BSA Free [NBP2-37397]

Immunocytochemistry/ Immunofluorescence: ALDH2 Antibody (4G6A3) - BSA Free [NBP2-37397]

Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (4G6A3) [NBP2-37397] - Immunofluorescence analysis of HepG2 cells using ALDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: ALDH2 Antibody (4G6A3) - BSA Free [NBP2-37397]

Immunohistochemistry: ALDH2 Antibody (4G6A3) - BSA Free [NBP2-37397]

Immunohistochemistry: ALDH2 Antibody (4G6A3) [NBP2-37397] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ALDH2 mouse mAb with DAB staining.

Applications for ALDH2 Antibody (4G6A3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:75

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH2

ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq]

Long Name

Aldehyde Dehydrogenase 2 Family Member

Alternate Names

ALDHI, ALDM

Gene Symbol

ALDH2

Additional ALDH2 Products

Product Documents for ALDH2 Antibody (4G6A3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ALDH2 Antibody (4G6A3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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