ALDH4A1 Antibody (002)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90152
Recombinant Monoclonal Antibody
![Western Blot: ALDH4A1 Antibody (002) [NBP2-90152]](https://resources.bio-techne.com/images/products/ALDH4A1-Antibody-002-Western-Blot-NBP2-90152-img0004.jpg "Western Blot: ALDH4A1 Antibody (002) [NBP2-90152]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 002
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human ALDH4A1 (Accession#: AAH07581.1; Lys25-Gln563).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ALDH4A1 Antibody (002)
Western Blot: ALDH4A1 Antibody (002) [NBP2-90152]
Western Blot: ALDH4A1 Antibody (002) [NBP2-90152] - Anti-ALDH4A1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L(Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 62 kDa Observed band size: 62 kDa (We are unsure as to the identity of these extra bands.)![Immunoprecipitation: ALDH4A1 Antibody (002) [NBP2-90152]](https://resources.bio-techne.com/images/products/ALDH4A1-Antibody-002-Immunoprecipitation-NBP2-90152-img0003.jpg "Immunoprecipitation: ALDH4A1 Antibody (002) [NBP2-90152]")
Immunoprecipitation: ALDH4A1 Antibody (002) [NBP2-90152]
Immunoprecipitation: ALDH4A1 Antibody (002) [NBP2-90152] - ALDH4A1 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate. Lane B:0.5 mg K562 Whole Cell Lysate. 2 uL anti-ALDH4A1 rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-ALDH4A1 rabbit monoclonal antibody,at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa.Applications for ALDH4A1 Antibody (002)
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ALDH4A1
Alternate Names
aldehyde dehydrogenase 4 family, member A1, Aldehyde dehydrogenase family 4 member A1, ALDH4DKFZp779M035, delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial, EC 1.5.1.12, mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase, P5C dehydrogenase, P5CD, P5CDh
Gene Symbol
ALDH4A1
Additional ALDH4A1 Products
Product Documents for ALDH4A1 Antibody (002)
Product Specific Notices for ALDH4A1 Antibody (002)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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