ALDH5A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52099

Novus Biologicals, part of Bio-Techne
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NBP1-52099
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ALDH5A1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SQDPAQIWRVAE corresponding to internal region according to NP_001071.1.

Epitope

C-SQDPAQIWRVAE

Specificity

This antibody is expected to recognize both reported isoforms (NP_733936.1; NP_001071.1). Amino acid numbering in name calculated from NP_733936.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ALDH5A1 Antibody

Western Blot: ALDH5A1 Antibody [NBP1-52099]

Western Blot: ALDH5A1 Antibody [NBP1-52099]

Western Blot: ALDH5A1 Antibody [NBP1-52099] (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-52099]

Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-52099]

Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-52099] - (5g/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for ALDH5A1 Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in human liver lysates (calculated MW of 58.7 kDa band according to NP_733936.1). Please note that both this product and EB10436 detects a 50 kDa band in mouse and rat liver lysates (calculated MW of 56.0 kDa band according to mouse NP_766120.1). IHC-P: Human liver shows heavy textured cystoplasm staining in hepatocytes, consistent with mitochondia.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ALDH5A1

This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

aldehyde dehydrogenase 5 family, member A1, EC 1.2.1, EC 1.2.1.24, NAD(+)-dependent succinic semialdehyde dehydrogenase, SSADHmitochondrial

Entrez Gene IDs

7915 (Human); 214579 (Mouse); 291133 (Rat)

Gene Symbol

ALDH5A1

UniProt

NP_733936.1 (Human)

Additional ALDH5A1 Products

Product Documents for ALDH5A1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ALDH5A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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