ALDH5A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52099

Novus Biologicals, part of Bio-Techne
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NBP1-52099
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ALDH5A1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SQDPAQIWRVAE corresponding to internal region according to NP_001071.1.

Epitope

C-SQDPAQIWRVAE

Specificity

This antibody is expected to recognize both reported isoforms (NP_733936.1; NP_001071.1). Amino acid numbering in name calculated from NP_733936.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ALDH5A1 Antibody

Western Blot: ALDH5A1 Antibody [NBP1-52099]

Western Blot: ALDH5A1 Antibody [NBP1-52099]

Western Blot: ALDH5A1 Antibody [NBP1-52099] (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-52099]

Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-52099]

Immunohistochemistry-Paraffin: ALDH5A1 Antibody [NBP1-52099] - (5g/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for ALDH5A1 Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in human liver lysates (calculated MW of 58.7 kDa band according to NP_733936.1). Please note that both this product and EB10436 detects a 50 kDa band in mouse and rat liver lysates (calculated MW of 56.0 kDa band according to mouse NP_766120.1). IHC-P: Human liver shows heavy textured cystoplasm staining in hepatocytes, consistent with mitochondia.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ALDH5A1

This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

aldehyde dehydrogenase 5 family, member A1, EC 1.2.1, EC 1.2.1.24, NAD(+)-dependent succinic semialdehyde dehydrogenase, SSADHmitochondrial

Entrez Gene IDs

7915 (Human); 214579 (Mouse); 291133 (Rat)

Gene Symbol

ALDH5A1

UniProt

NP_733936.1 (Human)

Additional ALDH5A1 Products

Product Documents for ALDH5A1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ALDH5A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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