ALDH7A1 Antibody (105)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89927

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-89927-100ul
NBP2-89927-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 105

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ALDH7A1 Antibodies »

Product Summary for ALDH7A1 Antibody (105)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ALDH7A1 (Uniprot#: P49419-2; Ser 2-Gln 511).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ALDH7A1 Antibody (105)

Western Blot: ALDH7A1 Antibody (105) [NBP2-89927]

Western Blot: ALDH7A1 Antibody (105) [NBP2-89927]

Western Blot: ALDH7A1 Antibody (105) [NBP2-89927] - Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane. SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:58 kDaObserved band size:58 kDa
Immunoprecipitation: ALDH7A1 Antibody (105) [NBP2-89927]

Immunoprecipitation: ALDH7A1 Antibody (105) [NBP2-89927]

Immunoprecipitation: ALDH7A1 Antibody (105) [NBP2-89927] - Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-ALDH7A1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ALDH7A1 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size: 55 kDa

Applications for ALDH7A1 Antibody (105)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH7A1

Antiquitin (Aldh7a1) is an evolutionarily conserved protein believed to play a role in the regulation of cellular turgor. Based on sequence analysis, this protein is classified as a member of the aldehyde dehydrogenase superfamily (1). Analysis of the amount of mRNA in various rat and human tissues indicates that the largest amounts are found in rat kidney and liver and in cultured human hepatoma cells. Only minimal amounts were detected in human peripheral blood leukocytes, rat lung, or cultured human fibroblasts (2). The plant homolog of ATQ1 is thought to be involved in regulating turgor pressure, a function that also would be essential for cells of the mammalian cochlea. Northern blots of 13 human fetal tissues show antiquitin to be highly expressed in cochlea, ovary, eye, heart, and kidney (3).

Alternate Names

aldehyde dehydrogenase 7 family, member A1, Aldehyde dehydrogenase family 7 member A1,26g turgor protein homolog, Alpha-AASA dehydrogenase, ATQ1Antiquitin-1, Betaine aldehyde dehydrogenase, Delta1-piperideine-6-carboxylate dehydrogenase, EC 1.2.1, EC 1.2.1.3, EC 1.2.1.31, EC 1.2.1.8, EPDalpha-aminoadipic semialdehyde dehydrogenase, FLJ11738, FLJ92814, P6c dehydrogenase, PDEantiquitin-1

Gene Symbol

ALDH7A1

Additional ALDH7A1 Products

Product Documents for ALDH7A1 Antibody (105)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ALDH7A1 Antibody (105)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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