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Aldo-keto Reductase 1C3/AKR1C3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1940

Novus Biologicals, part of Bio-Techne
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NB100-1940

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence CFASHPNYPYSDEY corresponding to C-Terminus according to NP_003730.4.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aldo-keto Reductase 1C3/AKR1C3 Antibody

Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940]

Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940]

Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940] - Staining of human breast cells lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940]

Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940]

Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940] - Staining of paraffin embedded human small intestine. Antibody at 5 ug/mL.Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Aldo-keto Reductase 1C3/AKR1C3 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01 - 0.1 ug/mL
Application Notes
WB: Approx. 35 kDa band observed in human liver and human breast lysates (calculated MW of 36.9 kDa band according to NP_003730).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aldo-keto Reductase 1C3/AKR1C3

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Long Name

Aldo-keto Reductase Family 1, Member C3

Alternate Names

AKR1C3, AldoketoReductase 1C3, DD3, DDX, HA1753, HAKRB, HSD17B5, PGFS

Entrez Gene IDs

8644 (Human)

Gene Symbol

AKR1C3

UniProt

Additional Aldo-keto Reductase 1C3/AKR1C3 Products

Product Documents for Aldo-keto Reductase 1C3/AKR1C3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aldo-keto Reductase 1C3/AKR1C3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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