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ALG2 Antibody (OTI3C2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01734

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3C2

Concentration

0.82 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human ALG2(NP_149078) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALG2 Antibody (OTI3C2)

Western Blot: ALG2 Antibody (OTI3C2) [NBP2-01734]

Western Blot: ALG2 Antibody (OTI3C2) [NBP2-01734]

Western Blot: ALG2 Antibody (OTI3C2) [NBP2-01734] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ALG2 monoclonal antibody.
Immunohistochemistry-Paraffin: ALG2 Antibody (OTI3C2) [NBP2-01734]

Immunohistochemistry-Paraffin: ALG2 Antibody (OTI3C2) [NBP2-01734]

Immunohistochemistry-Paraffin: ALG2 Antibody (OTI3C2) [NBP2-01734] - Staining of paraffin-embedded Human prostate tissue using anti-ALG2 mouse monoclonal antibody.
Western Blot: ALG2 Antibody (OTI3C2) [NBP2-01734]

Western Blot: ALG2 Antibody (OTI3C2) [NBP2-01734]

Western Blot: ALG2 Antibody (OTI3C2) [NBP2-01734] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2.

Applications for ALG2 Antibody (OTI3C2)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.82 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ALG2

This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. [provided by RefSeq].

Alternate Names

alpha-1,3-mannosyltransferase ALG2, alpha-1,3-mannosyltransferase), asparagine-linked glycosylation 2 homolog (S. cerevisiae, asparagine-linked glycosylation 2 homolog (yeast, asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S.cerevisiae), Asparagine-linked glycosylation protein 2 homolog, CDGIi, EC 2.4.1, EC 2.4.1.-, FLJ14511, GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase, hALPG2, homolog of yeast ALG2, NET38

Entrez Gene IDs

85365 (Human)

Gene Symbol

ALG2

Additional ALG2 Products

Product Documents for ALG2 Antibody (OTI3C2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ALG2 Antibody (OTI3C2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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