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ALG2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84569

Novus Biologicals, part of Bio-Techne
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NBP1-84569
NBP1-84569-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LYTPSNEHFGIVPLEAMYMQCPVIAVNSGGPLESIDHSVTGFLCEPDPVHFSEAIEKFIREPSLKATMGLAGRARVKEKFSPEAFTEQLYRYVTK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALG2 Antibody

Western Blot: ALG2 Antibody [NBP1-84569]

Western Blot: ALG2 Antibody [NBP1-84569]

Western Blot: ALG2 Antibody [NBP1-84569] - Analysis in control (vector only transfected HEK293T lysate) and ALG2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: ALG2 Antibody [NBP1-84569]

Immunohistochemistry-Paraffin: ALG2 Antibody [NBP1-84569]

Immunohistochemistry-Paraffin: ALG2 Antibody [NBP1-84569] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Applications for ALG2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALG2

ALG2 encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Alternate Names

alpha-1,3-mannosyltransferase ALG2, alpha-1,3-mannosyltransferase), asparagine-linked glycosylation 2 homolog (S. cerevisiae, asparagine-linked glycosylation 2 homolog (yeast, asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S.cerevisiae), Asparagine-linked glycosylation protein 2 homolog, CDGIi, EC 2.4.1, EC 2.4.1.-, FLJ14511, GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase, hALPG2, homolog of yeast ALG2, NET38

Entrez Gene IDs

85365 (Human)

Gene Symbol

ALG2

UniProt

Additional ALG2 Products

Product Documents for ALG2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ALG2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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