ALG2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85715
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KFLLLSINRYERKKNLTLALEALVQLRGRLTSQDWERVHLIVAGGYDERVLENVEHYQELKKMVQQSDLGQYVTFLRSFSDKQKISLLH
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ALG2 Antibody
![Western Blot: ALG2 Antibody [NBP1-85715]](https://resources.bio-techne.com/images/products/ALG2-Antibody-Western-Blot-NBP1-85715-img0004.jpg "Western Blot: ALG2 Antibody [NBP1-85715]")
Western Blot: ALG2 Antibody [NBP1-85715]
Western Blot: ALG2 Antibody [NBP1-85715] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue![Immunocytochemistry/ Immunofluorescence: ALG2 Antibody [NBP1-85715]](https://resources.bio-techne.com/images/products/ALG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85715-img0005.jpg "Immunocytochemistry/ Immunofluorescence: ALG2 Antibody [NBP1-85715]")
Immunocytochemistry/ Immunofluorescence: ALG2 Antibody [NBP1-85715]
Immunocytochemistry/Immunofluorescence: ALG2 Antibody [NBP1-85715] - Staining of human cell line U-2 OS shows localization to cytosol & actin filaments. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: ALG2 Antibody [NBP1-85715]](https://resources.bio-techne.com/images/products/ALG2-Antibody-Immunohistochemistry-Paraffin-NBP1-85715-img0002.jpg "Immunohistochemistry-Paraffin: ALG2 Antibody [NBP1-85715]")
Immunohistochemistry-Paraffin: ALG2 Antibody [NBP1-85715]
Immunohistochemistry-Paraffin: ALG2 Antibody [NBP1-85715] - Staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.Applications for ALG2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ALG2
Alternate Names
alpha-1,3-mannosyltransferase ALG2, alpha-1,3-mannosyltransferase), asparagine-linked glycosylation 2 homolog (S. cerevisiae, asparagine-linked glycosylation 2 homolog (yeast, asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S.cerevisiae), Asparagine-linked glycosylation protein 2 homolog, CDGIi, EC 2.4.1, EC 2.4.1.-, FLJ14511, GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase, hALPG2, homolog of yeast ALG2, NET38
Gene Symbol
ALG2
Additional ALG2 Products
Product Documents for ALG2 Antibody
Product Specific Notices for ALG2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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